Metabolomics reveals the response of hydroprimed maize to mitigate the impact of soil salinization

Zhang, Enying, Zhu, Xinglian, Wang, Wenli, Sun, Yue, Tian, Xiaomin, Chen, Ziyi, Mou, Xinshang, Zhang, Yanli, Wei, Yueheng, Fang, Zhixuan, Ravenscroft, Neil, O'Connor, David, Chang, Xianmin and Yan, Min (2023) Metabolomics reveals the response of hydroprimed maize to mitigate the impact of soil salinization. Frontiers in Plant Science, 14. ISSN 1664-462X

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Abstract

Soil salinization is a major environmental stressor hindering global crop production. Hydropriming has emerged as a promising approach to reduce salt stress and enhance crop yields on salinized land. However, a better mechanisitic understanding is required to improve salt stress tolerance. We used a biochemical and metabolomics approach to study the effect of salt stress of hydroprimed maize to identify the types and variation of differentially accumulated metabolites. Here we show that hydropriming significantly increased catalase (CAT) activity, soluble sugar and proline content, decreased superoxide dismutase (SOD) activity and peroxide (H2O2) content. Conversely, hydropriming had no significant effect on POD activity, soluble protein and MDA content under salt stress. The Metabolite analysis indicated that salt stress significantly increased the content of 1278 metabolites and decreased the content of 1044 metabolites. Ethisterone (progesterone) was the most important metabolite produced in the roots of unprimed samples in response to salt s tress. Pathway enrichment analysis indicated that flavone and flavonol biosynthesis, which relate to scavenging reactive oxygen species (ROS), was the most significant metabolic pathway related to salt stress. Hydropriming significantly increased the content of 873 metabolites and significantly decreased the content of 1313 metabolites. 5-Methyltetrahydrofolate, a methyl donor for methionine, was the most important metabolite produced in the roots of hydroprimed samples in response to salt stress. Plant growth regulator, such as melatonin, gibberellin A8, estrone, abscisic acid and brassinolide involved in both treatment. Our results not only verify the roles of key metabolites in resisting salt stress, but also further evidence that flavone and flavonol biosynthesis and plant growth regulator relate to salt tolerance.

Item Type:	Article
Keywords:	land degradation, soil salinization, hydropriming, progesterone, metabolomics analysis
Depositing User:	Professor Xianmin Chang
Date Deposited:	09 Jun 2023 10:26
Last Modified:	09 Jun 2023 10:26
URI:	https://rau.repository.guildhe.ac.uk/id/eprint/16576

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