Transcriptomic analysis for differential expression of genes involved in secondary metabolite production in Narcissus pseudonarcissus field derived bulb and in vitro callus

Chang, Xianmin, Ferdausi, A and Jones, M (2021) Transcriptomic analysis for differential expression of genes involved in secondary metabolite production in Narcissus pseudonarcissus field derived bulb and in vitro callus. Industrial Crops and Products, 168. ISSN 0926-6690

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Abstract

The Amaryllidaceae genus Narcissus contains diversified secondary metabolites, which are important sources of biologically active alkaloids. The biochemical pathways for secondary metabolite production require primary metabolites to undergo a series of modifications catalyzed by several enzymes but the knowledge on their biosynthesis is very limited. This study is undertaken to reveal the differential expression of transcripts related to secondary metabolites biosynthesis in Narcissus basal plate and in vitro callus. RNA-seq analysis of Narcissus basal plate (field) and in vitro callus ensued total 2153 differentially expressed transcripts and 83.46 % of those were annotated. The largest gene group was designated as uncharacterized proteins (10.24 %) and the second largest group was genes responsible for secondary metabolite production (8.88 %). The genes involved in the biosynthesis of alkaloids; cytochrome P450s, O-methyltransferases (OMTs), NADP/NADPH dehydrogenases or reductases, SAM-synthetases or decarboxylases, 3-ketoacyl-CoA, acyl-CoA, cinnamoyl-CoA, cinnamate 4-hydroxylase, alcohol dehydrogenase, caffeic acid, N-methyltransferase, and NADPH-cytochrome P450s were present in both basal plate and callus. However, cytochrome P450s, and OMTs which are responsible for regulating the later stage of alkaloids biosynthesis were mainly up-regulated in field samples. Whereas, the enzymes involved in initial biosynthetic pathways, fructose biphosphate adolase (FBA), aminotransferases, dehydrogenases, hydroxyl methyl glutarate (HMT), and glutamate synthase (GS) leading to the biosynthesis of precursors of secondary metabolites; tyrosine, phenylalanine, and tryptophan were up-regulated in callus. Furthermore, Gene Ontology (GO) annotation of the transcripts and associated Kyoto Encyclopedia of Genes and Genomes (KEGG) and Plant Reactome pathway maps were postulated. This knowledge on probable genes or enzymes involved in secondary metabolism, their networking pathway and molecular regulation in field and in vitro callus would provide a deep insight into the Narcissus plant biology related to valuable metabolite production.

Item Type:	Article
Additional Information:	12 month embargo taken from Journal Embargo List for UK Authors
Keywords:	NarcissusRNA-seqSecondary metabolismAlkaloidsGene expression
Divisions:	Agriculture, Food and Environment
Depositing User:	Professor Xianmin Chang
Date Deposited:	17 May 2021 09:21
Last Modified:	19 Oct 2022 14:44
URI:	https://rau.repository.guildhe.ac.uk/id/eprint/16453

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