Characterization of the extracellular proteases from Bacillus inaquosorum strain E1‐8 and its application in the preparation of hydrolysates from plant and animal proteins with antioxidant, antifreeze and anti‐browning properties

Liu, Zhiyun, Lin, Huawei, Zhu, Xiaolong, Wu, Xueying, Wu, Chenxi, Obajemihi, Obafemi Ibitayo, Liu, Xinyi, Su, Wenrui, Liu, Guangchao, Li, Yang, Xu, Xingfeng, Yang, Jie and Sun, Qingjie (2024) Characterization of the extracellular proteases from Bacillus inaquosorum strain E1‐8 and its application in the preparation of hydrolysates from plant and animal proteins with antioxidant, antifreeze and anti‐browning properties. Journal of the Science of Food and Agriculture. pp. 1-12. ISSN 0022-5142

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Abstract

BACKGROUND: Bacillus inaquosorum strains is widely recognized for their plant-growth-promoting and biocontrol capabilities, yet their roles in protease production remain unclear. This study aims to comprehensively assess the protease-producing performance of B. inaquosorum strain E1-8, while also exploring the novel application of agricultural Bacillus proteases in the preparation of protein hydrolysates for fresh-cut fruits preservation. RESULTS: Firstly, genomic sequencing revealed the diversity of E1-8 proteases, indicating 15 putative extracellular proteases. Subsequently, the fermentation conditions for E1-8 protease production were optimized, with sweet potato powder and soybean meal identified as the most suitable carbon and nitrogen sources, respectively, resulting in a maximum protease activity of 321.48 U/ml. Upon culturing the strain under these optimized conditions, only an S8 family serine protease and an M48 family metalloprotease were revealed by secretomic analysis and protease inhibitor assays. Additionally, the optimal protease conditions for generating protein hydrolysates from soy, pea, fish, and porcine proteins were determined. The molecular weight of the hydrolysates primarily ranged from 2000 to 180 Da, with a total of 17 amino acids identified. The application of these hydrolysates demonstrated a DPPH scavenging activity ranging from 58.64% to 84.12%, significantly reducing of the melting peaks and the freezing points. Furthermore, the browning index of apple slices stored at 4oC decreased by 14.81% to 22.15% on the second day, and similar effects were observed in fresh-cut banana stored at 4oC for 7 days. CONCLUSION: The protein hydrolysates obtained exhibit remarkable antioxidant, antifreeze, and anti-browning properties for fresh-cut fruits. Keywords: agricultural microorganism; extracellular proteases; animal and plant protein; protein hydrolysates; fresh-cut fruits; fresh-keeping activity

Item Type:	Article
Additional Information:	"This is the peer reviewed version of the following article: [Liu, Z., Lin, H., Zhu, X., Wu, X., Wu, C., Obajemihi, O.I., Liu, X., Su, W., Liu, G., Li, Y., Xu, X., Yang, J. and Sun, Q. (2024), Characterization of the extracellular proteases from Bacillus inaquosorum strain E1-8 and its application in the preparation of hydrolysates from plant and animal proteins with antioxidant, antifreeze and anti-browning properties. J Sci Food Agric. https://doi.org/10.1002/jsfa.13879], which has been published in final form at https://doi.org/10.1002/jsfa.13879. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited."
Keywords:	agricultural microorganism, extracellular proteases, animal and plant protein, protein hydrolysates, fresh-cut fruits, fresh-keeping activity
Divisions:	Land and Property Management
Depositing User:	Dr Obafemi Obajemihi
Date Deposited:	14 Oct 2024 13:07
Last Modified:	14 Oct 2024 13:07
URI:	https://rau.repository.guildhe.ac.uk/id/eprint/16790

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