The Effect of Moxidectin Treatment on the Equine Hind Gut Microbiome, Metabonome and Feed Fermentation Kinetics in Horses with Very Low Parasite Burdens

Daniels, S, Leng, J, Ellis, R, Swann, J, Moore-Colyer, M and Proudman, C (2017) The Effect of Moxidectin Treatment on the Equine Hind Gut Microbiome, Metabonome and Feed Fermentation Kinetics in Horses with Very Low Parasite Burdens. Equine Veterinary Education, 29 (58). p. 6. ISSN 2042-3292

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Abstract

Seventeen horses, mean 12 ± 3.5 years, kept at pasture, with haylage provided and no concentrates. Faecal Egg Counts were conducted September 2015‐March 2016, no eggs seen, no anthelmintic given. Sampling commenced March 2016, points were 0 (prior), 16, 48 and 168 hours post anthelmintic. Treatments were randomized, nine animals dosed orally with Moxidectin 18.92 mg/g at 0.4 mg/kg bw and eight controls. Three horses from each group were randomly assigned for fermentation kinetics. Sequencing of the 16S rRNA gene was conducted on extracted faecal bacterial DNA, bioinformatics using QIIME assigning operational taxonomic units (OTUs). LEfSe (Segata et al., 2011) was used to identify differentially abundant OTUs. Bacterial metabolic profiles were characterized by 1H NMR spectroscopy (Escalona et al., 2015), from urine, analysed by Principal Components Analysis. Fermentation of hay and oats, separately, were measured by in vitro gas production (Murray et al., 2006), data were analysed by repeated measures ANOVA.

Item Type: Article
Additional Information: Special Issue: Abstracts from the 12th International Equine Colic Research Symposium, July 18‐20, 2017, Hyatt Regency, Lexington, Kentucky, USA, July 2017
Keywords: Moxidectin Treatment, Equine Hind Gut Microbiome, Metabonome, Feed Fermentation Kinetics, Parasite Burdens
Divisions: Equine Management and Science
Depositing User: Marieke Guy
Date Deposited: 20 Feb 2019 21:01
Last Modified: 22 May 2019 15:02
URI: https://rau.repository.guildhe.ac.uk/id/eprint/16115

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